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Experiment details for rarg

Neofunctionalization in vertebrates: the example of retinoic acid receptors.

Neofunctionalization in vertebrates: the example of retinoic acid receptors.

Gene Clone Species Stages Anatomy
rarg.S laevis NF stage 10 to NF stage 11 marginal zone , animal hemisphere
rarg.S laevis NF stage 12 ectoderm , circumblastoporal collar , dorsal
rarg.S laevis NF stage 14 ectoderm , circumblastoporal collar , unspecified , anterior , posterior , [+]
rarg.S laevis NF stage 18 chordal neural plate , anterior , posterior , preplacodal ectoderm , anterior placodal area , [+]
rarg.S laevis NF stage 25 hyoid crest , pharyngeal arch , mandibular arch , tail bud , cranial placode , [+]
rarg.S laevis NF stage 29 and 30 to NF stage 32 otic vesicle , somite , pharyngeal arch , mandibular arch , hyoid arch , [+]

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  Figure S5. Expression of xRARα, xRARβ, and xRARγ in Xenopus embryos Whole mounts are oriented with anterior toward the right. Expression of RARα and RARγ is detectable from the onset of gastrulation (stage 10) while the first signal for RARβ is detected at the early tailbud stage (stage 25). At mid-gastrula stage (stage 11), RARα is expressed as a narrow ring around the blastopore. As gastrulation proceeds, expression intensifies and the signal around the blastopore widens preferentially on the dorsal side except in the midline, which exhibits a low level of transcripts. During neurulation (from stage 14), transcripts are found predominantly in the neurectoderm, evenly distributed along the anterior-posterior axis, with the exception of a region at the anterior end for which transcripts are largely reduced. At the tailbud stage (stage 30), RARα is predominantly expressed in the spinal cord and the posterior hindbrain, in the eye and the posterior branchial arches. During gastrulation (stage 10), expression of RARγ is more widespread than RARα expression. Transcripts are present in the mesodermal marginal zone as well as in the ectoderm. By the neurula stage (from stage 14), the staining separates into anterior and posterior domains, thus creating a gap with no RARγ transcripts. Expression remains localized to the posterior and anterior ends of the embryo at tailbud stages (stage 30) and is mainly restricted to the branchial arches and the tip of the tailbud. RARβ transcripts are detected at much lower level than RARα and RARγ at the examined stages. The signal is restricted to the caudal part of the hindbrain and the anterior spinal cord. At the late tailbud stage (stage 32), RARβ is strongly expressed in the most posterior branchial arches. d, dorsal views; l, lateral views; f, frontal views. doi:10.1371/journal.pgen.0020102.sg005

Gene Clone Species Stages Anatomy
rarg.S laevis NF stage 29 and 30 pharyngeal arch , mandibular arch , hyoid arch , branchial arch , tail bud , [+]

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  Figure 5. Schematic Representation of the Expression Territories of RARs Staining of embryos indicates expression of mRARα (A), mRARβ (B), and mRARγ (C) in mouse embryos at E9; of xRARα (G), xRARβ (H), and xRARγ (I) in stage 30 Xenopus embryos, and of AmphiRAR (M) in 20 h old amphioxus larvae. Schematic representations are shown of the expression territories of mRARs (D�F), xRARs (J�L), and AmphiRAR (N) in mouse, Xenopus, and amphioxus embryos, respectively. Regions with high levels of expression are red and those with lower levels of expression are pink. Arrowheads indicate regions in mouse and Xenopus embryos where the RAR expression cannot be correlated with AmphiRAR expression and can be described as �new expression territories.� doi:10.1371/journal.pgen.0020102.g005