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Fig. 2. A–D) frontal views of tadpole faces showing whole mount in situ hybridizations of RALDH2 (A,C) and RARγ (B,D) mRNA expression. The cement gland (cg) is pseudo-colored brown. The arrow points to the stomodeum. E,F) Transverse sections of RARγ mRNA expression and neural crest marker AP-2 in the orofacial region. G,H) DiI (red) fate mapping and phalloidin counterstaining labeling all F-actin (green). G) A frontal view of a tadpole fixed immediately post labeling with DiI in the RARγ expression domain at stage 28 (corresponding to panel D). The arrow points to the stomodeum. H) DiI labeling in transverse section approximately 40 h post labeling. DiI is observed in the primary palate region dorsal to the oral cavity. Abbreviations: cg; cement gland. |
