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Fig. 6. The effects of X-Su(H)1, X-Su(H)1/Ank and X-Su(H)1DBM on primary neurogenesis. Albino Xenopus embryos were injected once at the two-cell stage with test RNAs (1.0 ng/embryo) along with lacZ RNA (0.2 ng/embryo). At neural plate stages, the embryos were processed both for b-galactosidase expression (light blue) to mark the injected side, and for the expression of N-tubulin (dark purple) to mark
the formation of primary neurons. Primary neurons arise in three stripes on either side of the midline of the neural plate: a long medial stripe, a short more anterior intermediate stripe, and a wide lateral stripe. Shown are dorsal views, with anterior to the left and the injected side oriented up in each panel. Embryos shown in each row are from the same experiment. Expression of: (A) wild-type X-Su(H)1 has little or no
effect on the formation of primary neurons; (B) X-Su(H)1/Ank abolishes primary neurons; (C,F) X-Su(H)1DBM produces a neurogenic phenotype; (D) X-Su(H)1 along with X-Su(H)1DBM ameliorates the neurogenic phenotype; (E) X-Su(H)1/Ank along with X-Su(H)1DBM completely reverses the neurogenic phenotype; (G)X-Delta-1 reduces the number of primary neurons; (H) X-Su(H)1 along with X-Delta-1 partially
reverses the effects of X-Delta-1; and (I) X-Su(H)1DBM along with X-Delta-1 not only reverses the effects of X-Delta-1 but also results in a neurogenic phenotype. |
