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rmnd5axenopus   

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Experiment details for rmnd5a

Pfirrmann T et al. (2015) Assay

RMND5 from Xenopus laevis is an E3 ubiquitin-ligase and functions in early embryonic forebrain development.

Gene Clone Species Stages Anatomy
rmnd5a.L laevis NF stage 3 (4-cell) to NF stage 4 (8-cell) animal blastomere , animal , animal pole
rmnd5a.L laevis NF stage 18 ectoderm , ciliated epidermal cell , neuroectoderm , eye , head
rmnd5a.L laevis NF stage 24 ciliated epidermal cell , neuroectoderm , somite , eye , head
rmnd5a.L laevis NF stage 33 and 34 forebrain , somite , intermediate mesoderm , eye , cranial neural crest , [+]

  Fig 2. rmnd5 is expressed during early embryonic development. (A) Temporal RT-PCR analysis of rmnd5 expression (top panel); different developmental stages (NF-stages) indicated at the top. ODC1 functions as RNA input control (bottom). (B) Rmnd5 protein at different developmental stages. Western blot analysis of embryo lysate from indicated stages (top). α-RMND5A (Novus Biological; rabbit, 1:1000); α-Tubulin (AbD Serotec, rat, 1:2500). (C) Spatial analysis of rmnd5 expression. Whole mount in situ hybridisation (Wmish) of wild type Xenopus laevis embryos at different developmental stages. NF stage 3 (panel a, left) and stage 4 (panel c) rmnd5 transcript in the animal pole (top), NF-stage 12 (panel d) rmnd5 transcripts around the prospective head, NF-stage 18; 24 (panel e, f, g, h) neuronal ectoderm (red arrow, panel e) and ciliated cells of the skin (yellow arrow, panel e, g, h), NF-stage 34 (panel j, k, l, m) proencephalon (red arrow) and eyes (green arrow). Negative controls with sense probes (panel b, i).

Gene Clone Species Stages Anatomy
rmnd5a.L laevis NF stage 4 (8-cell) animal blastomere , animal , animal pole

  rmnd5a (required for meiotic nuclear division 5 homolog A ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 4, lateral view.

Gene Clone Species Stages Anatomy
rmnd5a.L laevis NF stage 18 ciliated epidermal cell , neuroectoderm , eye , cranial neural crest

  rmnd5a (required for meiotic nuclear division 5 homolog A ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 18, antereo-lateral view

Gene Clone Species Stages Anatomy
rmnd5a.L laevis NF stage 24 ciliated epidermal cell , neuroectoderm , head

  rmnd5a (required for meiotic nuclear division 5 homolog A ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, lateral view, anterior left, dorsal up.

Gene Clone Species Stages Anatomy
rmnd5a.L laevis NF stage 33 and 34 spinal cord , pharyngeal arch , mandibular arch , hyoid arch , branchial arch , [+]

  rmnd5a (required for meiotic nuclear division 5 homolog A ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 33 & 34 lateral view, anterior left, dorsal up.