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sema5bxenopus   

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Experiment details for sema5b

Koestner U et al. (2008) Assay

Semaphorin and neuropilin expression during early morphogenesis of Xenopus laevis.

Gene Clone Species Stages Anatomy
sema5b.S laevis NF stage 11 to NF stage 12 axial mesoderm
sema5b.S laevis NF stage 14 to NF stage 15 notochord , posterior , preplacodal ectoderm
sema5b.S laevis NF stage 16 to NF stage 19 notochord , prechordal plate , neural plate , posterior , preplacodal ectoderm
sema5b.S laevis NF stage 23 to NF stage 27 notochord , tail region , eye , paraxial mesoderm , anterior dorsal lateral plate region
sema5b.S laevis NF stage 28 to NF stage 32 notochord , tail region , optic vesicle , paraxial mesoderm , pronephric kidney , [+]

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  Figure 4. Expression of Sema5B and the class-6 semaphorins in Xenopus laevis. ba, branchial arches; e, eye; no, notochord; op, otic placode; p, pronephros; pnc, premigratory neural crest cells; s, somites.

Gene Clone Species Stages Anatomy
sema5b.S laevis NF stage 13 notochord

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  Figure 5. Semaphorin expression in the mesoderm and notochord during neurula stages. A-G: Neurula stage embryos were stained by in situ hybridization and transversally sectioned. Left: Whole embryo, dashed line indicates the plane of the transverse section. Right: Enlargement of the neural tube area of the transverse section. A: Sema3A is expressed in the somitogenic mesoderm. B: Sema4B expression delineates the boundaries of the somitogenic mesoderm. C: Sema6B is expressed in the somitogenic mesoderm. D: Sema3F is expressed in the notochord as well as the spinal cord anlage and the archenteron roof. E-G: Sema4C, Sema4D, and Sema5B are expressed in the notochord. arc, archenteron roof cells; m, somitogenic mesoderm; no, notochord; sca, spinal cord anlage.

Gene Clone Species Stages Anatomy
sema5b.S laevis NF stage 32 brain , midbrain , hindbrain , neural crest , eye , [+]

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  Figure 7. Semaphorins are expressed in close proximity to migrating cranial neural crest cells. In situ hybridization of tadpole-stage embryos; enlargements of the head are shown. Red arrows indicate migrating neural crest cells, white arrows indicate neurogenic placodes. A: Sema3A expression. Staining in the midbrain hindbrain boundary was confirmed by double in situ hybridization with engrailed (data not shown). B: Sema3C expression. C: Sema3D expression. D: Sema3F expression. Double in situ hybridization using Krox20 or engrailed antisense probes was used to confirm staining in the rhombomeres or the midbrain hindbrain boundary, respectively (data not shown and Q). E: Sema4A expression. F: Sema4B expression. G: Sema4C expression. H: Sema4G expression. I: Sema5B expression. K: Sema6A expression. L: Sema6B expression. M: Sema6D expression. N: Sema3A expression at early tadpole stage. O: Double in situ hybridization using a Sema3A (blue) and a twist (red) antisense probe at tadpole stage. Sema3A expression is localized posterior to the migrating twist-expressing neural crest cells (black arrow). P: For comparison twist expression at tadpole stage is shown. Q: Double in situ hybridization showing Sema3F (blue) expression adjacent to the anterior portion of the migrating Krox20-expressing (red) neural crest cells (black arrow). cg, cement gland; e, eye; mh, midbrain hindbrain boundary; op, otic placode; r3, rhombomere 3; r5, rhombomere 5.

Gene Clone Species Stages Anatomy
sema5b.S laevis NF stage 37 and 38 spinal cord , head region , branchial arch , pronephric kidney

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  Figure 6. Semaphorin expression in the mesoderm and notochord at tadpole stages. In situ hybridization of the whole embryo (left), dashed line indicates the plane of the transverse section (right). A: Sema3A is expressed in the somites and the splanchnic mesoderm. B: Sema4B expression in the mesoderm is replaced by pronephros, cement gland, otic placode, proctodeum, spinal cord, and gut expression. C: Sema6B is expressed in the somites and otic placode at late neurula stages. D: At tadpole stages, the somite expression is limited to the dorsal and lateral margins of the somites (white arrow). E: Sema3F expression is detected in the notochord, but also in the spinal cord, the hypochord, the posterior endoderm, and the branchial arches. F: Sema4C notochord expression is replaced by expression in the posterior cardinal vein, the heart, and the branchial arches. G: Sema4D expression is detected in the notochord. H: Sema5B expression in the notochord is replaced by spinal cord, pronephros, brain, and eye expression. ba, branchial arches; cg, cement gland; en, endoderm; g, gut; h, heart; hc, hypochord; no, notochord; op, otic placode; p, pronephros; pcv, posterior cardinal vein; pd, proctodeum; s, somites; sc, spinal cord; sm, splanchnic mesoderm.