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Experiment details for set

A requirement for NF-protocadherin and TAF1/Set in cell adhesion and neural tube formation.

A requirement for NF-protocadherin and TAF1/Set in cell adhesion and neural tube formation.

Gene Clone Species Stages Anatomy
set.S laevis NF stage 13 pre-chordal neural plate , neural plate
set.S laevis NF stage 15 pre-chordal neural plate , chordal neural plate , presomitic mesoderm , neural plate , paraxial mesoderm , [+]
set.S laevis NF stage 17 pre-chordal neural plate , chordal neural plate , neural crest , presomitic mesoderm , neural plate , [+]
set.S laevis NF stage 20 presomitic mesoderm , posterior neural tube , paraxial mesoderm , anterior neural tube , cranial neural crest , [+]
set.S laevis NF stage 28 brain , forebrain , midbrain , hindbrain , somite , [+]

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  Fig. 1. Xenopus TAF1 and NFPC expression in the neural ectoderm. In situ hybridization analysis for TAF1 (A�E, I�M) or NFPC (F�H, N, O), viewed in wholemount (A�I) or in cross section (J�O). At stage 13, TAF1 is expressed throughout the neural plate (np), with highest expression anteriorly. At stages 15 (B) and 17 (C), TAF1 is expressed in the neural folds (nf). After neural tube closure, TAF1 is expressed throughout the neural tube (nt), in the eye (ey) brachial arches (ba), and forebrain (fb) (D, E). In contrast, at stage 17, NFPC expression is restricted to the tips of the neural folds (arrow in panels F and G) and in the ventral ectoderm. After neural tube closure, NFPC is also expressed in the anterior neural tube, eye, and branchial arches (H). (I) A stage 17 embryo stained by in situ for TAF1 (purple) and with an antibody to NFPC (brown) demonstrates co-localization of NFPC and TAF1 at the tips of the neural folds (arrow). Sections through embryos reveal that TAF1 is expressed throughout the neural plate, the somatic mesoderm (sm), and the inner layer of the epidermal ectoderm (J�M), while NFPC is localized to the tips of the neural folds (arrow in panel N) and the inner layer of the epidermal ectoderm prior to neural fold fusion. After the neural tube has closed, NFPC is expressed in the ventral neural tube (O). Other abbreviations: nc, notochord; psm, presomitic mesoderm.