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FIG. 2. The temporal and spatial expression of XSmad4a and
XSmad4b. A, XSmad4a and XSmad4b transcripts are present during
Xenopus early embryogenesis. Equivalent amounts of total RNA isolated
from each stage of embryos were analyzed for the expression of
XSmad4a and XSmad4b in an RNase protection assay. Numbers represent
the developmental stages (st.) (32): stages 1 and 6, maternal;
stage 11, gastrula; stage 16, neurula; stage 23, tailbud; stages 28, 34,
and 39, tadpole. The expression of ornithine decarboxylase (ODC) was
also examined as a control for equal loading of RNA. B–G, the spatial
expression patterns of XSmad4a and XSmad4b in developing Xenopus
embryos are analyzed by whole mount in situ hybridization: B and E,
animal view of early gastrula stage embryos (stage 10, ventral, at the
top); C and F, dorsal view of neurula stage embryos (stage 20, anterior
to the left); D and G, lateral view of tadpoles (stage 31, anterior to the
left). |
