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smad4.2xenopus otic vesicle [+] 

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Experiment details for smad4.2

Masuyama N et al. (1999) Assay

Identification of two Smad4 proteins in Xenopus. Their common and distinct properties.

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Gene Clone Species Stages Anatomy
smad4.2.S laevis NF stage 31 otic vesicle

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  FIG. 2. The temporal and spatial expression of XSmad4a and XSmad4b. A, XSmad4a and XSmad4b transcripts are present during Xenopus early embryogenesis. Equivalent amounts of total RNA isolated from each stage of embryos were analyzed for the expression of XSmad4a and XSmad4b in an RNase protection assay. Numbers represent the developmental stages (st.) (32): stages 1 and 6, maternal; stage 11, gastrula; stage 16, neurula; stage 23, tailbud; stages 28, 34, and 39, tadpole. The expression of ornithine decarboxylase (ODC) was also examined as a control for equal loading of RNA. B–G, the spatial expression patterns of XSmad4a and XSmad4b in developing Xenopus embryos are analyzed by whole mount in situ hybridization: B and E, animal view of early gastrula stage embryos (stage 10, ventral, at the top); C and F, dorsal view of neurula stage embryos (stage 20, anterior to the left); D and G, lateral view of tadpoles (stage 31, anterior to the left).