| |
Fig. 3. Expression of TN transcripts in tailbud stage-29/30 embryos. Transverse sections. (A, B, D, E, F) Hybridizations with the
antisense probe. (C) Control hybridization with the sense probe. (A, B, C) Anterior truncal level; (A) heart primordium region; (B, C)
level posterior to A. TN transcripts are principally detected in the somitic mesoderm. Transcription of TN mRNA also occurs at a lower
level in the pericardial mesoderm (arrowheads). No hybridization is observed in the sense control. (D, E, F) Posterior levels : high levels
of TN mRNA are expressed in somites and in cells dispersed around neural tube and notochord (arrowheads). TN transcripts are also
detected in the basal layer of the tailfin epidermis (arrows) and in cells close to the postanal gut (large arrowhead). No hybridization can
be seen in lateral plate mesoderm. Scale bar = 250 mm. |
