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Figure 7.
Colocalization of TH2 and TPH1 in the PVO of chicken and Xenopus. Frontal sections obtained from the PVO of chicken (a–d) and Xenopus (e–l) are shown. TH2 (a; magenta) and TPH1 (b; green) transcripts are observed in the same region of the chicken PVO, and some CSF-c cells coexpress both genes (c, d; white). The area depicted by a dashed rectangle in (c) is shown in (d1–d3) at higher magnification. Optical sectioning by confocal microscopy shows that TH2 (d2) and TPH1 (d3) are expressed in the soma (arrowheads) and in the processes of CSF-contacting cells (arrow). Orthogonal views of optical sections confirm the overlap of TH2 and TPH1 signals. In Xenopus, th2 (e, i; magenta) and tph1 (f, j; green) are expressed both in the rostromedial (e–h) and the caudolateral (i–l) parts of the PVO. Some CSF-c cells coexpress both genes (g, k). The coexpression is confirmed by orthogonal views of optical sections (h1) of the area depicted by a dashed rectangle in (g), showing that th2 (h2) and tph1 (h3) are colocalized in the same cells (arrowheads). D = dorsal; L = lateral; LR = lateral recess; v = ventricle. Scale bar = 100 µm in (a) (applies to a, b, c) and in (e) (applies to e, f, g); 50 µm in (i) (applies to i, j, k, l) |
