| |
Fig. 2. Darkfield photomicrographs illustrating the localization of
TRH precursor mRNA hybridization signal in selected regions of the
brain of white-adapted Xenopus laevis. Brain sections were dipped
into Kodak NTB-2 liquid emulsion and exposed for 2 weeks. A: In the
medial septum (ms), a dense accumulation of silver grains was seen
over numerous cell bodies. B: In the anterior preoptic area (Poa), a
strong hybridization signal was visualized in cells lining the ventricle.
C: The highest density of silver grains was observed in the magnocellular
preoptic nucleus (Mg) and the suprachiasmatic nucleus (SC),
with dense accumulation of proTRH mRNA signal over cell bodies.
D: Control section, consecutive to that in C, incubated with a sense
riboprobe. E: In the ventral hypothalamic nucleus (VH), many cells
were densely covered by silver grains. F: In the mesencephalic tectum
(tect), scattered cells were strongly labeled. G: In the nucleus reticularis
medius (Rm), a moderate density of silver grains was seen over
a few cells. Scale bar 100 m. |
