| |
Fig. 2. ADAM19 mRNA distribution. Whole mount in situ hybridization using a probe corresponding to Xenopus ADAM19. (A) Lateral view of a bisected gastrula with the dorsal side to the left and the animal pole at the top (ap). (B and G) Dorsal views of early tailbud stage embryos with the anterior at the top. (C–E, I) Lateral views of tailbud stage embryos with anterior to the left. (F) Ventral view of an early tailbud stage embryo. Photographs in (A, F and I) were taken with embryos in PBS to visualize superficial rather than internal staining. All other embryos were imaged in Murray’s clear. (A) ADAM19 is first expressed in the dorsal mesoderm of the gastrula, with the most intense signal in the dorsal blastopore lip (dbl). (B, F–H) At early tailbud stage, ADAM19 is found in the dorsal mesoderm including the notochord (n), somites (s), the cement gland (cg) and the neural tube (nt). (C, D and I) ADAM19 expression in these tissues persists during tailbud formation. In the anterior, ADAM19 is expressed in the brain (br), the otic (ot) and optic (o) vesicles as well as CNC segments and branchial arches (ba). (E) At stage 36, the expression in the pronephros (p) is also detected. |
