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Figure 1. Determination of the expression pattern of ARVCF by whole-mount in situ hybridization. X. laevis embryos at different developmental stages were examined using an antisense probe directed against ARVCF. a: Lateral view of stage-18 embryo with dorsal side at the top, anterior to the left. The arrowhead shows enriched transcripts in the neural plate and bordering neural crest. b: Lateral view of a stage-26 tailbud with the anterior towards the left. The line shows the position of the section shown in panel c. c: Horizontal section through the head region of a stage-26 tailbud showing the presence of ARVCF transcripts in the cranial neural crest cells (yellow arrowhead). d: A similar section of a stage-26 embryo stained with the neural crest marker Twist as a reference. e: Stage-31 early tadpole showing enriched ARVCF expression in the head region and the heart (yellow arrowhead).Download figure to PowerPoint |
