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Figure 4. xBmal1 is expressed from neural plate to late tailbud stages.
Shown are in situ hybridization results depicting expression of xBmal1 mRNA. All embryos are oriented with the anterior to the left in all panels except A and D. Panel A shows the embryo from the anterior, but slightly angled to one side. D shows an anterior view. Panels C, F, H and J show side views of the embryos. Panels B, E G, and I show dorsal views of the embryos. Panels K and O show transverse sections and panel N shows a sagittal section of late tailbud embryos. Panel A and B depict a stage 18 embryo with xBmal1 staining in the neural plate and cement gland (blue arrow). C show stage 23 (neural tube stage) embryos with expression in the eye (black arrow), cement gland (blue arrow), and somites (red arrowhead). F show early (F) and late (H) tailbud stages where xBmal1 is expressed in the eye, pineal (orange arrowhead), otic vesicle (black arrowhead), somites (red arrowhead) and the pronephric tubules and duct (green arrows). Cement gland staining was lost (blue arrow). K and L show expression in the brain, retina/lens, pineal (orange arrowhead), and absence of staining in the cement gland (blue arrow). M show expression in the olfactory pit (green arrowhead), otic vesicle (black arrowhead), pronephric tubules (green arrow), heart (blue arrowhead), somites (red arrowhead), and notochord (orange arrow). No expression was seen using a sense probe specific to xBmal1 (J). |
