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Figure 2.
RNG105 is highly expressed in dendrites of hippocampal neurons. A, Western blotting of extracts (30 μg each) from Xenopus tissues and A6 cells with anti-XRNG105 antibody. Arrows denote XRNG105. In brain and A6 cells, XRNG105 was detected as doublets, although the reasons for this are not known. B, Immunostaining of slices from the lateral pallium of Xenopus forebrain with anti-XRNG105 antibody. A schematic drawing is shown on the left. Scale bar, 50 μm. C, Rat brain extracts were immunoblotted with an antibody directed against mammalian RNG105. Arrow, RNG105. D, Slices from rat hippocampus (top) and cerebellum (bottom) were immunostained with anti-RNG105 antibody (green) and costained with anti-MAP2 antibody, which locates dendrites (red). RNG105 was concentrated in the dendritic region of the stratum radiatum (SR) and in the nuclei of pyramidal neurons [stratum pyramidale (SP)] in the hippocampus. In contrast, RNG105 was not detectable in the dendrites of Purkinje cells in the molecular layer (ML) of the cerebellum. Merge, Merged images; SO, stratum oriens; GL, granule cell layer; PL, Purkinje cell layer. Scale bars, 50 μm. E, Rat cultured hippocampal neurons were immunostained with anti-RNG105 antibody (top) and costained with anti-MAP2 antibody (bottom). Bottom, Composite with a differential interference contrast (DIC) image. Arrowheads indicate dendrites; arrows indicate an axon. Scale bar, 10 μm. |
