|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gadd45g
|
|
laevis
|
NF stage 41
to
NF stage 42
|
retina
,
ciliary marginal zone
,
eye
|
elavl3
|
|
laevis
|
NF stage 41
to
NF stage 42
|
retina
,
ciliary marginal zone
,
eye
,
retinal inner nuclear layer
,
retinal ganglion cell layer
,
[+]
|
ebf3
|
|
laevis
|
NF stage 41
to
NF stage 42
|
retina
,
ciliary marginal zone
,
eye
,
retinal ganglion cell layer
,
retinal ganglion cell
|
atoh7
|
|
laevis
|
NF stage 41
to
NF stage 42
|
retina
,
ciliary marginal zone
,
eye
|
vxn
|
|
laevis
|
NF stage 41
to
NF stage 42
|
retina
,
ciliary marginal zone
,
eye
|
|
|
Fig. 5. Proneural bHLH target genes are expressed in late progenitors or post-mitotic neurons of the X. laevis retina. (A) Schematic of stage 41/42 X. laevis retinal cross-section. The central retina contains post-mitotic cells stratified into 3 major layers: retinal ganglion cell (RGC) layer, inner nuclear layer (INL) and outer nuclear layer (ONL). The ciliary marginal zone (CMZ) contains retinal stem cells within the most peripheral region (per) and early differentiating progenitors within the central region (cntl). The entire neural retina is surrounded by retinal pigment epithelium (RPE). (B) In situ hybridizations were performed on paraffin sections of stage 41/42 X. laevis embryos. (B) Xath5 expression is restricted to the central CMZ (bracket) and excluded from the most peripheral CMZ. (C) SBT1, Xgadd45γ and XETOR, respectively, are also restricted to progenitors and early differentiating neurons within the central CMZ (bracket). (F) elrC is expressed in differentiating neurons in the most central CMZ region (arrow in panel F) and persists in post-mitotic cells of the RGC layer and a subset of cells within the INL. (G) Xebf3 expression is also present in differentiating neurons of the central CMZ (arrow in panel G) and maintained in mature RGCs. |