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Figure 5. N-Cadherin-Dependent Contacts Are Required for Collective Chemotaxis(A–F) N-cadherin expression in premigratory (A–C) and migratory (D–F) NC cells analyzed by whole mount in situ hybridization (A and D) and immunostaining (B, C, E, and F); NC cells streams are delimited by dotted lines.(G and H) N-cadherin loss-of-function using an antisense Morpholino (n = 87).(I and J) Full-length N-cadherin overexpression (n = 40).(K–S) NC cells labeled with rhodamine-dextran (RD) were grafted into unlabeled embryos and NC migration was monitored looking at the RD fluorescence. Immunostaining on sections for N-cadherin (N and O), β-catenin (P and Q), and p120-catenin (R and S) are shown in low and high magnification. Blue, DAPI staining. Scale bar, 20 μm.(T–V) Tracks of control cells ([J], n = 16) and NC pretreated with a control IgG ([K], n = 22) or with N-cadherin blocking antibody NCD2 ([L], n = 27) and exposed to Sdf1 showing that N-cadherin inhibition strongly blocks chemoattraction toward Sdf1. Chemotaxis index for each condition is shown in (W). b, branchial; h, hyoid. Error bars show standard deviation. See also Movies S7 and S8. |
