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Fig. 4. Localization of XPolycomb and Xbmi-I transcripts during early Xenopus development by whole mount in situ hybridization. Developing embryos at stage 9 late blastula (A,E), stage 15 early neurula (B,F), stage 19 late neurula (C,G) and stage 20-28 tailbud (D,H) were hybridized with antisense XPolycomb (A-D) and Xbmi-I (E-H) probes. All pictures are lateral views. The blastocoel (b) (A,E) and the anterior regions (a) of the neurula embryos (B,C,F,G) are indicated. In D the focus is on the hybridization signals in the spinal cord (top) or in the somites (bottom). Specific staining in the rhombencephalon (r) and spinal cord (sp.c) (D,H) and somites (s) (D) is indicated. |