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Fig. 2. Spatial analysis of Clock mRNA expression in Xenopus retina. Xenopus eyes were dissected and fixed at ZT 2 and 14 μm cryosections were prepared. In situ hybridization analysis was done with digoxygenin-labeled antisense (left) or sense (right) XClock riboprobes. The white arrowhead emphasizes the heavy in situ labeling in the inner segments of the photoreceptor cells. Retinal cell layers are labeled on the left: RPE, retinal pigment epithelium; OS, photoreceptor outer segments; ONL, outer nuclear layer (photoreceptor cell nuclei); INL, inner nuclear layer; and GCL, ganglion cell layer. |