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Fig. S5. Barhl2 or Casp3 depletion phenotypic defects. (A and B) Disorganization of the neuroepithelium. Representative IHC for β-catenin (red; membrane staining) together with P-3H (red; nuclear staining) merged with BB (cyan) images on diencephalic sections of st 26/27 Xenopus embryos injected with MO- barhl2 (A) or MOcasp3 (B). The (Aa) control and (Ab) injected sides of representative sections are shown. Inj, injected side. Arrows indicate part of the apical surface where β-catenin staining is abnormal; arrow end indicates mitotic nuclei. (C and D) Increase in Wnt signaling in the prosomere P2 alar plate ccnd1 expression profiles at st 33 or 30 analyzed by ISH. The ct (Ca) and injected (Cb) sides of one representative dissected neural tube flat-mounted and (Cc) a section at the diencephalic level are shown. The percent of embryos exhibiting the phenotype is indicated. (Ca) ct and (Cb and Cc) Barhl2-depleted (89%, n = 19); (Da) ct and (Db and Dc) Casp3-depleted neural tubes (82%, n = 39). The white rectangle delineates the prosomere P2 alar plate. The black arrow indicates lo- calization of the optic stalk. |
