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Figure 3. Spatial expression patterns of Xenopus tropicalis DAZ-like gene (Xtdazl) mRNA until neurula stage by whole-mount in situ hybridization. A,B: Section of Xtdazl-stained oocyte showed that Xtdazl mRNA was expressed in the perinuclear region in a stage (St.) I oocyte (A) and (B) in the vegetal pole of a stage III oocyte. C: Section of St. 4 embryo stained with Azan. Arrows indicate vegetal pole cytoplasm. D-I: Spatial distribution of Xtdazl mRNA in a fertilized egg (St.1, D), eight-cell stage embryo (St. 4, E), late blastula (St. 9, F), gastrula (St. 10.5, G), early neurula (St.13, H), and late neurula (St. 18. I). J-L: Section of St.1 (J), St.4 (K), and St. 9 (L) embryo with staining for Xtdazl. Animal side is up. M-O: Sagittal section of Xtdazl-stained embryo at the gastrula stage (St. 10.5, M), early neurula stage (St.13, N), and late neurula stage (St. 18, O). Scale bars = 100 mu m in A,B, 200 mu m in C,J-O, 500 mu m in D-I. |
