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Fig. 4. Expression patterns of ET3. A,C: ET3 is expressed in the rostral trunk region, where melanophores later migrate to the yolk at stages 25 (A) and 31 (B). B,D: ET3 is expressed in thecaudal trunk region where melanophores later localize in the NT. E,F: Dct expression represents
migrating melanoblasts. E and F are serial sections of C and D, respectively. In the rostral region, melanoblasts seek yolk migrating through the ventral pathway (E). In the caudal region, melanoblasts localize in the NT or migrate to the fin (F). Scale bar ¼ 100 mm. |
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Fig. 2. Expression patterns of Ednrb2 and Dct A–D: Serial sections of an embryo at stage 26.
Dct-expressing cells also express Ednrb2 in the rostral trunk region (A, B) and in the caudal
trunk region (C, D). A melanoblast initiating migration is observed in A, B (arrowheads). E,F:
Both Dct (E) and Ednrb2 (F) are expressed in the same cells in serial sections of the trunk region
at stage 32. G,H: RPE cells express Dct (G) but not Ednrb2 (H) in serial sections of the eye at
stage 32. Note that the brown color of H in the RPE is not the ISH signal but is due to the melanin.
Scale bar ¼ 100 mm. |
