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Figure 4. ATP4 Is Required for Canonical and Noncanonical Wnt Signaling(A) Phenotypes of ATP4a morphant tadpoles, which were less pigmented, displayed shortened AP axes, small heads, and reduced eyes.(B and C) Canonical Wnt signaling. (B) Reduced En2 expression at the mid-hindbrain boundary of (bottom) morphant stage 26 tadpole. (C) Xwnt8- and Dvl2-mediated, but not β-cat-induced, twinning requires ATP4a.(D) Noncanonical Wnt signaling. Convergent-extension movements of activin-induced animal cap explants: Reduced elongation in ATP4aMO-injected explants was rescued by coinjection of constitutively active (CA) RhoA. ∗∗∗Very highly significant. ns, not significant.See also Figure S3. |
