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Figure 2. In situ hybridization of six1+/− and six1 −/− X. tropicalis larvae. (a) Left image: pax2 expression in a six1+/− larva. Staining is strong in the otic vesicle (ov), retina (ret), and neural tube (nt). Right image: pax2 expression in a six1−/− larva is indistinguishable from the heterozygous sibling. (b) Left image: eya2 expression in a six1+/− larva. Staining is strong in ov, olfactory placode (olf), VII and IX/X cranial ganglia, nephric mesoderm (ne) and hypaxial muscle precursors (hyp). Middle image: eya2 expression in a six1−/− larva with a partial effect. ov (red arrow) and VIIg staining appears normal, ne and hyp staining appears reduced, and IX/Xg and olf staining is not detectable. Right image: eya2 expression in a six1−/− larva with a severe effect. Only ov staining (red arrow) is detected and ov is smaller than normal. (c) Left image: tbx1 expression in a six1+/− larva. Staining is strong in ventral ov and branchial arches (ba). Middle image: tbx1 expression in a six1−/− larva with a partial effect. ov (red arrow) and ba staining is reduced but not missing. Right image: tbx1 expression in a six1−/− larva with a complete loss of staining in the ov (red arrow) and ba. (d) Left image: dlx5 expression in a six1+/− larva. Staining is strong in ov, ba, and olf. Right image: Staining in ov (red arrow), ba and olf is not detected. (e) Left image: irx1 expression in a six1+/− larva. Staining is strong in ov and nt. Right image: irx1 expression in a six1−/− larva. Staining in ov (red arrow) and nt is not detected. (f) Left image: sobp expression in a six1+/− larva. Staining is strong in ov, VIIg (cg), olf, and nt. Right image: sobp expression in a six1−/− larva. Staining in ov (red arrow), cg, olf, and nt is not detected. (g) Left image: rnf150 expression in a six1+/− larva. Staining is strong in ov, ba, ret, and nt. Right image: rnf150 expression in a six1−/− larva. Staining in ov (red arrow), ret, and nt is not detected, and greatly reduced in ba. (h) Left image: pick1 expression in a six1+/− larva. Staining is strong in ov, ba, ret, and nt. Right image: pick1 expression in a six1−/− larva. Staining in ov (red arrow), ba, ret, and nt is not detected. All views are lateral with anterior to the right and dorsal to the top. |
