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Figure 2. FN Knockdown Retards Blastopore Closure, Slows Convergence and Extension, and Produces Tissue Thickening and an Excess of Actin-Rich Cellular Extensions in Whole Embryos
(A) Frames from a representative time-lapse sequence show that blastopore closure is delayed in 15 μMoles FNMO-injected embryos until sibling control embryos reach equivalent midgastrula stage 13.
(B) Anterior-posterior length to the mediolateral width ratios of the noninvoluting marginal zone of control morpholino-injected embryos (open circles), uninjected embryos (filled circles), or FNMO-injected embryos (filled triangles) show that FNMO-injected embryos lag 2 hr behind control embryos.
(C) Transverse confocal section through the dorsal axis of an embryo injected contralaterally with FNMO along with a FITC-dextran lineage tracer shows the absence of fibrils is accompanied by tissue thickening on the injected side.
(D and E) A maximal projection of a 30 μm thick sagittal confocal stack of a region just animal of the blastopore lip at stage 11.5 show that actin-rich cellular extensions are abundant in FNMO-injected embryos (D) but far less common within uninjected embryos (maximal projection of a 60 μm sagittal confocal stack; [E]). |
