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Figure 4. In Situ Hybridization of Vgl Probe to Oocytes, Eggs, and
Cleavage Embryos
Paraffin sections of an albino oocyte (A), an unfertilized egg (B and D),
and an early blastula embryo (C)were hybridized with 32P-labeled Vgl
(A, B, and C) or 32P-labeled histone H4 (D) antisense RNA probes.
The hybridization grains (white) show the location of Vgl or histone
mRNAs. Autoradiographic exposure was for 10 days (A and B), 2 weeks
(C), or 2 days(D). In each case, the animal (ectodermal) pole is at the
top and the vegetal (endodermal) pole is at the bottom. GV is the
oocyte’s nucleus or germinal vesicle. |
