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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
hand1	xenopus		foregut [+] acinar cell,alpha cell,antrum,beta cell,cardia,delta cell,distal stomach,epsilon cell,exocrine pancreas,fundus,gamma cell,islets of Langerhans,proximal stomach,pulmonary epithelium,pyloric sphincter,pylorus,stomach body,buccopharyngeal membrane,first pancreatic bud,islets of Langerhans,lung,lung bud,lung primordium,oral evagination,pancreas,pharyngeal cavity,pyloric sphincter,second pancreatic bud,stomach,stomach epithelium,taste bud,thyroid,thyroid primordium,ventral pancreatic bud,visceral pouch

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Experiment details for hand1

Asymmetrically reduced expression of hand1 homeologs involving a single nucleotide substitution in a cis-regulatory element.

Asymmetrically reduced expression of hand1 homeologs involving a single nucleotide substitution in a cis-regulatory element.

Gene	Clone	Species	Stages	Anatomy
hand1.L		laevis	NF stage 35 and 36	foregut

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Fig. 2. Developmental expression of hand1.L and hand1.S in Xenopus laevis. (A–K) The whole embryos showing expression at stages 15 (A and F), 17 (B and G), 19 (C and H), and 35/36 (D and I), and their high-magnification images (E and J). Cross-section showing heart at stage 35/36 (K). White arrowhead: epidermal cells; white arrow: anterior ventral portion; gray arrowhead: heart; black arrowhead: branchial arches. (L) RT-PCR analysis of hand1.L and hand1.S. The amplification efficiency of each primer pair was confirmed using genomic DNA from J-strain (5 ng, 1 ng, and 0.2 ng, respectively). RT-PCR was performed with cDNA from the heart tubes. Eukaryotic translation elongation factor 1 alpha 1/EF-1 alpha (eef1a.S) was used as internal control.