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Fig. 1. Comparison of the expression of XHRT1 and N-tubulin in neurula stage embryos and repression of XHRT1 expression by X-Ngnr-1. (A, B) Cross-sections in the posterior region of stage 17 embryos, XHRT1 stained (A) or XHRT1/N-tubulin double stained (B), showing that the expression of the two genes is exclusive. (C) Cross-section of a stage 16 embryo injected with X-ngnr-1 and stained with N-tubulin showing ectopic N-tubulin staining in the neural midline. (D) A X-ngnr-1 misexpressing embryo injected into the dorsal medial animal blastomeres at the 16-cell stage and a control embryo stained at stage 15 showing inhibition of XHRT1 expression in the injected area (15/17 embryos). |
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Gene |
Clone |
Species |
Stages |
Anatomy |
hey1
|
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laevis
|
NF stage 11
to
NF stage 12
|
ectoderm
,
dorsal
|
hey1
|
|
laevis
|
NF stage 13
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neural groove
,
neural plate
,
anterior
|
hes4
|
|
laevis
|
NF stage 11
|
ectoderm
,
neuroectoderm
|
hes4
|
|
laevis
|
NF stage 12
|
ectoderm
,
neuroectoderm
|
hes4
|
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laevis
|
NF stage 13
|
neural groove
,
neural plate
,
floor plate
,
neural plate border
|
hes4
|
|
laevis
|
NF stage 17
|
neural plate
,
sensorial layer of neurectoderm
,
floor plate
,
anterior neural fold
,
cranial neural crest
,
[+]
|
hes1
|
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laevis
|
NF stage 17
|
eye primordium
,
neural groove
,
neural plate
,
floor plate
,
pre-chordal neural plate border
,
[+]
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Fig. 6. Comparison of the expression of XHRT1, Xhairy1 and Xhairy2b in the midline of early Xenopus embryos. Embryos were analyzed by whole- mount in situ hybridization with probes for XHRT1 (A, C, E, G, I, K), Xhairy2b (B, D, F, H, J, L) and Xhairy1 (M, N). Stages of development are indicated on the left for each line of embryos. (A, B) Vegetal view with dorsal up, (C, I, J, M) dorsal view with anterior right, (G, H, K, L, N) transverse vibratome sections. Xhairy2b is activated earlier than XHRT1 in the developing floor plate and their expression overlaps extensively. Xhairy1 is weakly expressed in the neural midline. It is only detected starting from late neurula stage in the superficial layer of the prospective floor plate cells. |
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hey1 (hes-related family bHLH transcription factor with YRPW motif 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 17, transverse section, dorsal up. |