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Supplementry Fig. 2. (A) Vegetal view of a stage 11.5 embryo. The zygotic expression of Xenopus laevis Sulf1 is first detected in the mesoderm during mid-gastrula stage, but is excluded from the most dorsal mesoderm (arrowhead). (B) Dorsal view of a stage 14 embryo. Sulf1 is expressed in the paraxial mesoderm, but is excluded from the dorsal midline. (C) Lateral view of a stage 23 embryo. Sulf1 is expressed in the pronephros (arrowhead), somites and posterior mesoderm as well as anterior neural tissue. (D) Vegetal view of a stage 11.5 embryo hybridised to Xenopus laevis Sulf2. Zygotic Sulf2 expression is not detected. (E) Dorsal view of a stage 14 embryo. Zygotic Sulf2 expression is not detected. (F) Lateral view of a stage 23 embryo. Sulf2 expression is detected in a ventral region that could include haemangioblasts (arrowhead), in the mesendoderm underlying the cement gland (arrow), and in anterior neural tissue. (G) Vegetal view of a stage 11.5 embryo. The zygotic expression of Xenopus laevis6-O-sulfotransferase is only detected in endoderm as the punctuate staining indicated by the arrowhead. (H) Dorsal view of a stage 14 embryo. 6OST is expressed in the two bars in the presumptive hindbrain (arrowheads) and in the paraxial mesoderm. (I) Lateral view of a stage 23 embryo. 6OST is expressed in the endoderm (arrowhead) and in anterior neural tissues (arrow). |
