| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| kcnab3 | xenopus | subpallium [+] |
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Experiment details for kcnab3
Xenopus embryonic spinal neurons express potassium channel Kvbeta subunits.Xenopus embryonic spinal neurons express potassium channel Kvbeta subunits.
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| Fig. 2. xKvβ2 and xKvβ4 transcripts are present in excitable tissues of developing Xenopus embryos. Embryos were hybridized as whole mounts to antisense or sense cRNA probes corresponding to either xKvβ2 (A–F) or xKvβ4 (G–I). The in situ hybridization signal is recognizable as a deep blue-purple precipitate. The whole-mount embryos (A–C, G–I) are oriented with their dorsal sides up and anterior ends to theleft. Embryos that were hybridized to sense control probes are identified by s; these demonstrate the background signal (caused by trapping of probe in internal cavities), which is higher in older embryos. The transverse sections(D–F) are oriented dorsal sideup. A, At 26 hr (St 24), xKvβ2 mRNA is prominent in developing somites. B, Between 1 and 2 d (top, middle, and bottom embryos are St 28, 29, and 30, respectively), the pattern of expression of xKvβ2 mRNA undergoes a dramatic change. Initially the signal predominates in the somites but with time diminishes in this tissue and begins to appear in the spinal cord (arrowheads). At St 35–36 (50 hr; C), the signal is no longer observed in the somites but is present in the spinal cord and head. D, In a transverse section through a 1 d embryo hybridized to xKvβ2 antisense probe, the signal is present in the developing somites. E, In a transverse section of a 2 d embryo hybridized to xKvβ2 antisense probe, the signal is detectable in the dorsal spinal cord, where Rohon-Beard cells reside. F, In a transverse section of a 2 d embryo hybridized to a sense control probe, no signal is present.G–I, Whole-mount embryos hybridized to xKvβ4 antisense probe. At all stages, the signal predominates in the nervous system. In the older embryos (e.g., I), the signal is stronger. Comparison of H andI (xKvβ4) with B and C(xKvβ2) reveals that xKvβ4 mRNA localizes to a more ventral position in the spinal cord than does xKvβ2. Scale bar:A, G, 1 mm; B,C, 1.5 mm; H, 1.2 mm;I, 1.1 mm; D–F, 700 μm. | |||||||||||
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| kcnab3 (potassium channel, voltage gated subfamily A regulatory beta subunit 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up. |