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Figure 6. Kv2.2 protein was present early in the developing retina. Transverse cryostat (18 μm) sections were incubated with the Kv2.2 antibody or preblocked Kv2.2 antibody (inset). Sections were counterstained with Hoechst (blue). A,B: In the retina of St 41 embryos, Kv2.2 immunoreactivity localized to the inner and outer plexiform layers (ipl, opl), axons in the ganglion cell layer (gcl), and somata of the photoreceptor layer (prl). Kv2.2 was also present in the optic nerve (on) and occasional cell bodies and processes within the inner nuclear layer (inl). C: In the St 44 retina, Kv2.2 protein was present in the ganglion cell layer, inner and outer plexiform layers, photoreceptor layer somata, and a subset of somata and processes within the inner nuclear layer. D,E: In the St 47 retina, Kv2.2 protein showed a distribution similar to that found at St 44. Inset: Preincubation of the Kv2.2 antibody with Kv2.2 peptide blocked the immunoreactive signal. F: In the stage 50 retina, the Kv2.2 protein displayed less intense immunoreactivity in the inner plexiform and ganglion cell layers in comparison with previous stages. In other regions, the pattern of immunolabeling was similar to that of earlier stages. Scale bars = 20 μm in A–C,E,F; 200 μm in D.. |
