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Fig. 6. Activities of ERK1/2 and AKT in limb stumps. Immunostaining was performed with anti-diphospho-ERK1/2 (A–C) and anti-phospho AKT substrate (D–E) at 1 day (A, D), 2 days (B, E) and 4 days (C, F). All sections are longitudinal sections. (A, B) At 2 days after amputation, activity of ERK1/2 was detected in bundles of axons in the stump (arrowheads). (C) At 4 days, however, diphosphorylation of ERK1/2 was evident in mesenchymal cells under the WE as well as regenerating axons. (D) Activity of AKT was extensively detectable in the mesodermal region of the stump at 1 day. Activity level of AKT in the distal region was low (bracket). (E) Activity of AKT was more evident at 2 days. (F) At 4 days, activated AKT-positive cells became restricted to the distal region where the majority of mesodermal cells are GFP positive. Scale bars: 200 μm. |
