| |
Fig. 2. Whole-mount in situ hybridization using XMLP antisense RNA. Maternally expressed
XMLP was present in cleave stages (A,B). Afterwards signals were found in the ectoderm and
mesoderm of gastrula stages in (C,D). A sagittal section of stage11.5 is shown in (D). However,
at neurula stage, signals were no longer evenly distributed and were restricted to neural
ectoderm only (E,F,G). Subsequently, XMLP transcripts could be found in the neural folds at early
tailbud stage and presumptive brain area only (H,I,M). The sagital section of (I) is shown in (J)
and (K), while (L) shows a transversal section of (I). In later stages XMLP was expressed in
ectoderm derivatives and pronephros during stage 27 to 34, (N,O,Q). (P) shows a transversal
section of stage 34 (ac, archenteron; bt, blastocoel; bv, brain ventricle; ed, ectoderm; en,
endoderm; hm, head mesenchyme; mc, mesencephalon; md, mesoderm; nc, notochord; ne,
neural ectoderm; nt, neural tube; pn, pronephros; rc, rhombencephalon; sc, spinal chord). |
