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Gene/CloneSpeciesStageAnatomy ItemExperimenter
mmp14xenopus epidermis [+] 

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Experiment details for mmp14

Willson JA and Damjanovski S (2019) Assay



Gene Clone Species Stages Anatomy
mmp14.L laevis NF stage 20 epidermis
mmp14.L laevis NF stage 28 epidermis
mmp14.L laevis NF stage 33 and 34 epidermis
mmp14.L laevis NF stage 37 and 38 epidermis

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  Fig. 1. Localization of RECK and MT1-MMP proteins during early X. laevis development. Immunohistochemistry was performed using Alexa Fluor 555-labeled anti-RECK (red) and Alexa Fluor 488-labeled anti MT1-MMP (green) antibodies on stage 17–38 embryos. Brightfield DIC images are shown on the left panels. Mono-colour images for RECK and MT1-MMP are presented individually. Colocalization of RECK and MT1-MMP (yellow) is indicated on the right panels. Dotted line in stage 28 merged image indicates overlapping signal within the middle of somites. Dashed line in stages 34 and 38 merged images indicates overlapping signal along the ventral neural tube. Scale bar = 100 μm. Abbreviations: E = endoderm, Ep = epidermis, D = dorsal NT, DF = dorsal fin, N = notochord, NC = neural crest, NP = neural plate, NT = neural tube, S = somitic mesoderm, V = ventral NT.

Gene Clone Species Stages Anatomy
mmp14.L laevis NF stage 20 epidermis
mmp14.L laevis NF stage 28 epidermis
mmp14.L laevis NF stage 33 and 34 epidermis
mmp14.L laevis NF stage 37 and 38 epidermis

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  Fig. 3. Localization of TIMP-2 and MT1-MMP proteins during early X. laevis development. Immunohistochemistry was performed using Alexa Fluor 555-labeled anti-TIMP-2 (red) and Alexa Fluor 488-labeled anti MT1-MMP (green) antibodies on stage 17–38 embryos. Brightfield DIC images are shown on the left panels. Mono-colour images for TIMP-2 and MT1-MMP are presented individually. Colocalization of TIMP-2 and MT1-MMP (yellow) is indicated on the right panels. Dotted line in stage 28 merged image indicates overlapping signal within the middle of somites. Dashed line in stage 34 merged image indicates overlapping signal along the ventral neural tube. Scale bar = 100 μm. Abbreviations: E = endoderm, Ep = epidermis, D = dorsal NT, DF = dorsal fin, N = notochord, NC = neural crest, NP = neural plate, NT = neural tube, S = somitic mesoderm, V = ventral NT.