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Fig. 3. Whole-mount in situ hybridization showing the spatio-temporal expression pattern of Xseb-4. (A–J) Comparative expression analysis of Xseb-4 (A–E) and XmyoD (F–J) during gastrulation and neurulation. Note that Xseb-4 expression is embedded in the expression domain of XmyoD, but does not extend around the closing blastopore (red arrowheads). (K–M) Comparative expression of Xseb-4 (K), XmyoD (L) and Xnkx-2.5 (M) at stage 27. Additional expression commences in the lens vesicle and the heart. (O–Q) Transverse sections of a st. 32 embryo (N) from anterior to posterior direction through the lens (O), heart (P) and tailbud (Q). Expression in the heart is restricted to the myocardium. Abbreviations: le, lens; hm, head mesenchyme; ov, otic vesicle; m, myocardium; so, somites. |
