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Fig. 1.
The spatial–temporal expression pattern of NEDD4L during Xenopus embryonic development. (A) Schematic depiction of xNEDD4L encoding an evolutionarily conserved HECT E3 ligase closely related to NEDD4. The number of amino acid residues of each protein is indicated. (B) The expression of Xenopus NEDD4L during cleavage (2-cs), blastula (st8), gastrula (st10–12), neurula (st14–22) stages assessed by Western blot using a specific antibody directly against xNEDD4L. β-Actin serves as a loading control. (C–G) Whole mount in situ hybridization for xNEDD4L. (C) Animal view at st8; (C׳) lateral view at st8. (D) Dorsal view at st12 (bl: blastopore). (E) Dorsal view at st14; (E׳) anterior view at st14 (⁎ asterisk indicates anterior neural fold); (F) dorsal view at st16; (F׳) transversal section view at the level indicated by red dashed line in F (ar: archenteron); (G) lateral view at st24; (G׳), transversal section view at the level indicated by red dashed line in G. Scale bar: 1 mm. (H, I) Western blotting assessments of the distribution of NEDD4L protein in the early gastrula (st10.5, H) and late gastrula (st12, I). Embryos at st10.5 were dissected into animal (Ani) and vegetal (Veg) halves, or dorsal (Dor) and ventral (Ven) marginal zones. Embryos at st12 were dissected into dorsal (Dor) and ventral (Ven) marginal zones. β-Actin serves as a loading control. |
