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Figure 4.
Nocturnin Is Expressed in the Cytoplasm of Rod and Cone Photoreceptor Cells
(A) Eyecups from Xenopus laevis collected at ZT 8 and ZT 22 were sectioned and stained with anti-noc 1–20 antibody. For each time point, nocturnin staining is shown in the first photo, and a merged image of nocturnin staining (red) and nuclei stained with Hoescht dye (blue) is shown in the second photo. Nocturnin staining is photoreceptor specific and is higher at ZT 22 than at ZT 8. Exposure times are the same for all sections for comparison. Faint fluorescence in the inner retina is not consistently seen and may be due to long exposure time. PR, photoreceptor cell body; OPL, outer plexiform layer; INL, inner nuclear layer.
(B) A slide incubated without primary antibody serves as a negative control. |
