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Fig. 3. Nr2e3 and Tlx (Nr2e1) expression in retinal sections. In situ hybridization using paraffin sections of embryos at various stages during X. laevis development probed for Nr2e3 (A-C) and Tlx (Nr2e1) (D-F) expression. Nr2e3 is expressed in the neural retina, but not in the CMZ (A-C). Retinal expression of Tlx is restricted to the CMZ by stage 45 (arrows in (D-F). Retinal layers and the lens are labeled with a letter abbreviation: L, lens; G, ganglion cell layer; I, inner nuclear layer; P, photoreceptor layer. |
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Fig. 4. Co-expression of Nr2e3 and Rhodopsin in rods. (A) Double in situ hybridization for Nr2e3 (purple) and Rhodopsin (red) in a section of an eye from a stage 45 embryo. Arrows denote the overlap between Nr2e3 and Rhodopsin expression. (B) Higher magnification (optical and digital) of the picture in (A) showing the region of overlap between Nr2e3 and Rhodopsin expression (arrowhead). |