|
Display additional annotations [+]
|
| |
FIG. 4. Whole mount of the diencephalon and mesencephalon of tadpoles
(stage 51). (a) Retinal central pathways in a Xenopus tadpole.
All retina1 axons from the right eye were labeled with HRP to show
the projection to the visual centers, i.e., the optic tectum (OT), nucleus
Belonci (NB), corpus geniculatum thalamicum (CGT), and basal optic
nucleus (BON). OC: optic chiasm. For the method of HRP labeling,
see Fujisawa et ul. (1981). (b) The tadpole brain stained with MAb-A5
by the ABC method. The optic tectum, the nucleus Belonci, and the
corpus geniculatum thalamicum are positively stained. The staining
of the basal optic nucleus is obscure. (c) The tadpole brain stained
with MAb-B2 by the ABC method. All visual centers are negative
(X40). |
|
|
| |
FIG. 5. Indirect immunofluorescent analysis of the expression of A5-antigen in the optic tectum of eye-enucleated tadpoles (stage 51) in
which the right eye was enucleated at stage 29. The left tecta (*) was not innervated by optic nerves (X175). |
|
Display additional annotations [+]
|
| |
Fig.1 Indirect immunofluorescence of MAb-45 and MAb-B2 on cryostat sections of the optic tectum of Xenopus tadpoles (stage 53). (a) A coronal section of the mesencephalon stained with MAb-A5. Only the outer layers of the optic tectum (OT) show the fluorescence (arrow),
whereas the tegmentum (TG) is negative (X100). (b) A section similar to (a) stained with MAb-BX. Both the optic tectum and tegmentum are
stained. (c) Immunofluorescence with MAb-A5 in the optic tectum is restricted to the 8th and 9th plexiform layers (X160). (d) The section next
to (c) is stained with MAb-B2. The 3rd, 5th, and 7th plexiform layers of the optic tectum show immunofluorescence with MAb-B2, but the 8th
and 9th layers are practically negative. (e) The same section as (d) viewed with bright-field optics. The numbers indicate the 3rd, 5th, 7th, 8th,
and 9th plexiform layers of the optic tectum, respectively. |
|
Display additional annotations [+]
|
| |
FIG. 7. Immunofluorescent staining of viable tadpole brains with MAb-A5 (a) and MAb-B2 (b) (stage 53). Numbers indicate the 3rd, 5th, 7th,
8th. and 9th plexiform layers of the optic tectum (X140) |
|
|
| |
FIG. 8. MAb-A5 indirect immunofluorescent staining of trypsin-digested optic tectum (stage 53). (a) Intact optic tectum was treated with
0.05% trgpsin for 2 hr and then fixed and stained with MAb-A5 (b) Control nontrypsinized optic tectum stained with MAb-A5 (X100). |
