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Figure 1. Characterization of Xenopus PKMz. A, Alignment of predicted PKMz amino acid sequences from Xenopus laevis and other species. Regions corresponding to postulated major motifs are highlighted. B, Western blot using an antibody raised against the catalytic domain of rat PKMz identifies a 50 kDa protein from Xenopus brain extracts, demonstrates a progressive developmental increase in expression. C, Schematic drawing of tadpole brain (left) and horizontal section stained with PKMz antibody demonstrating enriched immunoreactivity in the neuropil region (NP) and the membrane portion of cell body (CB) region of the tectum. |
