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Fig. 2. Expression of XCoREST during Xenopus development. (A) At the beginning of neurulation (stage 13), XCoREST mRNA is localized in two stripes of cells at each side of the midline (arrowheads). (B) At stage 14, the expression of XCoREST is detected in three stripes (M, medial; I, intermediate and L, lateral stripes; arrowheads) at each side of the midline and in the trigeminal placode (tg). (C) N-tubulin is expressed in a similar three-stripe pattern (arrowheads). (D) Double in situ hybridization for XCoREST (purple) and N-tubulin (cian) mRNAs. (E) Upper panel shows a higher magnification of the stripe pointed at with an arrowhead in (D). Lower panel shows the same stripe of the same embryo after the first chromogenic reaction for N-tubulin detection (cian) and before the second chromogenic reaction for XCoREST detection (purple). (F–H) XCoREST expression at different neurula stages. (I–K) Lateral views. (I) At stage 21, XCoREST mRNA is also detected in the eye (arrowhead) and brain (arrow). This expression is also seen at later stages (J and K). (L) Double in situ for XCoREST (pink) and Xiro1 (cian) mRNAs. Note that XCoREST medial and intermediate stripes (arrowheads) are at the border of Xiro1 expression domains (arrow). (M–P) Hindbrain transverse sections of tailbud embryos showing XCoREST (M), Xngnr-1 (N), XDelta-1 (O) and N-tubulin (P) domains of expression (arrowheads). |
