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Fig. 5. ZIP12 is expressed primarily in the neural tube and brain of X. tropicalis and is required for neural tube closure and embryonic viability. (A and B) ZIP12 and β-actin or GAPDH expression was determined in various adult tissues and developmental stages by RT-PCR. (C) ZIP12 mRNA expression is elevated in the neural plate, as determined by quantitative RT-PCR (n = 6, +/- SE). ***P < 0.001 versus whole embryo; ###P < 0.001 versus rest of embryo. (D) ZIP12 mRNA (slc39a12) is expressed during neurulation and early nervous system development, analyzed by in situ hybridization. (E-M) ZIP12 knockdown by antisense morpholino microinjection impairs X. tropicalis development during neurulation. (N) Microinjection of slc39a12MO1 does not affect tubulin protein content, analyzed by immunoblotting. (O) Microinjection of slc39a12MO1 affects the ratio of polymerized to soluble β2-tubulin, as analyzed by polymerized tubulin fractionation and immunoblotting. (Scale bars: 250 μm in all images.) |
