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Fig. 3. Expression pattern of TGF-î² ligands during tail regeneration. (A) Whole-mount in situ hybridization with xTGF-î²2 antisense probe on regenerating tails at 0 hpa, 4 hpa, 8 hpa, 24 hpa, 2 dpa, and 3 dpa. Red arrows point to xTGF-î²2 expression (blue staining) in the regeneration bud. (B) Phase-contrast image at higher magnification of the 24 hpa tail from panel A, showing xTGF-î²2 expression (red arrow) in the regeneration bud proper and not in the overlying epithelium (black arrow). (C) Whole-mount in situ hybridization with xTGF-î²5 antisense probe on regenerating tails at 0 hpa, 2 hpa, 4 hpa, 24 hpa, 2 dpa, and 3 dpa, showing expression of xTGF-î²5 (red arrows) as early as 2 hpa. (D) Phase contrast image at higher magnification of the 2 dpa sample from panel C, showing xTGF-î²5 expression (red arrows) in the regeneration bud proper but not in the overlying epithelium (black arrow). Arrowheads: amputation plane. |
