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Fig. 9. Spatial pattern of expression of TSP-4 mRNA. Whole mount in situ hybridization was performed on albino Xenopus embryos using digoxygenin-UTP labelled antisense TSP-4 probes. All embryos are oriented with anterior to the right. A: Dorsal view of a stage 14 neurula, showing staining in the paraxial mesoderm, which flanks the unstained notochord (arrowhead). B: Stage 19 neurula, dorsal view. C: Side view of a stage 26 tailbud, arrowhead indicates segmented somite. D: Top view of a tailbud of similar stage to embryo in (C). E: Stage 33 tailbud, side view.
Arrow indicates progressive loss of staining from the somites. The faint blue staining in the gut is non-specific background. F: Stage 35 tadpole, side view. Arrowhead points to intense staining in the dorsal region of the somite and the arrow indicates peripheral skeletal muscle. G: Stage 39 tadpole, side view. Arrow indicates skeletal muscle staining. H: High magnification side view of the head of the embryo in (G). Arrow indicates stained muscles surrounding the eye. |
