| |
Fig. 4. Darkfield photomicrographs illustrating the localization of
xTRHR1 mRNA hybridization signal in selected regions of the brain of
white-adapted Xenopus laevis. Brain sections were dipped into Kodak
NTB-2 liquid emulsion and exposed for 1 month. A: In the internal
granular layer of the olfactory bulb (igl), labeled cells were observed
along the lateral ventricle (lv). B: A moderate hybridization signal
was seen in the striatum (Str) especially in the accumbens nucleus
(Acc). C: Control section, consecutive to that in B, incubated with a
sense riboprobe. D: The highest density of silver grains was observed
in the anterior preoptic nucleus (Poa), with a dense concentration of
xTRHR1 mRNA signal over cell bodies lining the ventricle. E: In the
nucleus of the paraventricular organ (Npv) and in the ventral hypothalamic
nucleus (VH), many cells showed a moderate density of
silver grains. F: A weak hybridization signal was detected in the
dorsal part of nucleus princeps of the trigeminal nerve (Vpr). 3v, Third
ventricle. Scale bar 100 m. |
