| |
Fig. 2. Injection of XSIP1 MO resulted in the down-regulation of terminal neural marker genes. Whole-mount in situ hybridization analysis of N-tubulin (A,B) and N-CAM (C,D) performed on stage 28 embryos. Embryos were injected with 40 ng of either control MO or XSIP1 MO into one dorsal-animal blastomere of the eight-cell stage, and co-injected with 250 pg of nuclear-localized lacZ as a lineage tracer (red stained). (A,C) Control MO-injected embryo. Injected area (red) and marker expression (blue) overlapped. (B, D) XSIP1 MO-injected embryo. Neural markers, N-tubulin and N-CAM were not expressed in the injected area. Inset; Dorsal view of the same embryos. |
