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Fig. 1. Comparison of the expression of XHRT1 and N-tubulin in neurula stage embryos and repression of XHRT1 expression by X-Ngnr-1. (A, B) Cross-sections in the posterior region of stage 17 embryos, XHRT1 stained (A) or XHRT1/N-tubulin double stained (B), showing that the expression of the two genes is exclusive. (C) Cross-section of a stage 16 embryo injected with X-ngnr-1 and stained with N-tubulin showing ectopic N-tubulin staining in the neural midline. (D) A X-ngnr-1 misexpressing embryo injected into the dorsal medial animal blastomeres at the 16-cell stage and a control embryo stained at stage 15 showing inhibition of XHRT1 expression in the injected area (15/17 embryos). |