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Fig. 3. Intracellular distribution of endogenous and exogenous XCS-1 protein. (A-C) Confocal images of whole mounts of stage 10 embryos
immunostained using the affinity purified anti-XCS-1 antibody to detect endogenous XCS-1. (A) Arrows are pointing to the XCS-1 protein
located on two structures that appear to be centrosomes; (B and C) different focal planes through the same blastomere showing centrosomes on
different sides of the nucleus; (D,E,H) in vitro-transcribed RNA from myc-XCS-1 (10 pg) was injected into fertilized eggs. Stage 10 embryos
were fixed and sectioned and immunostained with anti-myc antibody or anti-g-tubulin antibody. (D and E) Peroxidase-staining using anti-myc
antibody – arrows are pointing to the centrosomal area, the spindle and to the chromosomes on the spindle. (F) Confocal image of endogenous
XCS-1 protein decorating intracellular structures as well as cell membrane; (G) indirect immunofluorescence with the anti myc-XCS-1
antibody showing intracellular distribution of exogenous XCS-1 at the centrosomal area of 3 cells (arrows). (H) The same cells in panel G that
were immunostained with anti g-tubulin antibodies to show the position of the centrosomes (arrows). |
