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FIG. 1. AChR accumulation at sites of muscle contact with former neuritic pathways containing substrate-bound neural agrin. (A) Muscle
cells after 1 day in culture. (B) Agrin immunofluorescence. (C) AChR fluorescence. Similarly numbered arrows in B and C point to the same
s i t.es. AChR accumulated at sites where muscle cells contacted the agrin path ways. Arrow heads point to characteristic AChR patches on muscle
cells which did not contact agrin pathways. Bar, 25;;m. |
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FIG. 2. AChR accumulation confined to the borders of the agrin pathway. (A) Muscle cells after 1 day in culture. (B) Agrin immunofluorescence.
(C) AChR fluorescence. Almost all of the AChR accumulation is limited to the width of the agrin pathway. Bar, 5 .um. |
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FIG. 4. ChE accumulation along agrin pathways. (A) Agrin immunofluorescence. (B) AChR fluorescence . (C) ChE reaction product. The muscle
cells were cultured fo•· 2 days. ChE, like AChR, accumulated at sites where muscle cells contacted agrin pathways. Some ChE reaction product
i.s also seen elsewhere on the muscle ~ells. Bar, 1Q I'm. |
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FIG. 5. AChR accumulation along prestained agrin pathiVays. (Ai .Muscle cells after 1 day in culture. {B) Agrin immunofluorescence. The
immunofluorescent staining was carried out before the muscle cells were plated. (C) AChR fluorescence. Note the extensive accumulation of
AChR at sites where muscle cells contacted the prcstaincd agrin pathways. Ba r, 25J'm. |
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FIG. 6. AChR accumulation induced by ag•·in-positive, but not by agrin-negative, former neurit ic pathways. (A) Living SC neurons. After this
photograph was taken, the neurons were eliminated and muscle cells were plated. (B) F ixed muscle cells after 1 day in culture. (C) Agrin
immunofluor escence. Note that agrin was associated with only two of the former neuri tic pathways. (D) AChR fluorescence. The position of the
neurites before they were eliminated is indicated by the hlack lines. Note the extensive accumulation of AChR at sites wh<!re muscle cells
contacted agrin-positive pathways and the almost complete absence of AChR fluorescence where muscle cells contacted agri n-negative pathways.
Bar, 25 pm. |
