| |
Fig. 1. Expression of cdc45. Embryos in (A) as well as (F–H) are oriented anterior to the left and dorsal to the top. (A) Stage 13 embryo. Arrowheads demarcate
expression within the region which will become the sensory placodes. (B) Stage 13 embryo, anterior view with the dorsal side to the top. Arrowheads
demarcate higher intensity of expression within the anterior CNS and retinal rudiments. (C) Stage 22 embryo, dorsal view, anterior toward the top. Note dorsal
stain associated with the CNS with increased intensity anteriorly in the optic regions. Faint signal is also present in the posterior end of the embryo. (D) Stage
32 embryo, dorsal view, anterior toward the top. The bracket demarcates the right half of the CNS, and the left margin of the bracket aligns with the inner
boundary of the ventricular zone. Note the gradient of expression in the CNS. (E) Stage 37 embryo, dorsal view, anterior toward the top. Intense stain is located
in the ventricular region of the forebrain. (F) Stage 26 embryo. (G) Stage 32 embryo. (H) Stage 37 embryo. (I) Oblique section through the forebrain region of a
stage 37 embryo Note the intense stain in the ventricular zone of the forebrain as well as the ciliary marginal zone of the retina. cmz: ciliary marginal zone of
the retina, for: forebrain, lens: lens, mid: midbrain, nc: neural crest, oc: optic cup, ot: otic vesicle, ov: optic vesicle, pa: pharyngeal arches, phx: pharynx, vz:
ventricular zone. (J) Sense control stage 22 embryo. (K) Sense control stage 37 embryo. The scale bar in (H) equals 380 mm in (A and B), 760 mm in (C)
200 mm in (D) and (E), 550 mm in (F–H), (J and K), and 125 mm for (I). |
